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Dr Jaliya Kumaratilake  BVSc (University of Sri Lanka, Peradeniya, Sri Lanka),
PhD (Murdoch University, Murdoch, WA, Australia)
Senior Lecturer
e-mail: Jaliya.Kumaratilake@adelaide.edu.au Jaliya teaches gross anatomy, functional anatomy, clincal histology ( in particular human skin), fuctional microstructure ( in particular of connective tissues, bone cartilage, joints and lymphoid sysytem) and evolutionary aspects of the skin. He also teaches techniques in cytology (autoradiography, scanning electron microscopy, in situ hybridization) and immunohistochemistry. He is involved in teaching anatomy in Problem Based Learning (PBL) and Case Based Teaching (CBT). He has supervised 3 Honours Students, 2 MSc Students and 1 PhD Student. Research Interests:His research is in: - establishing immunoelectron microscopic methods (sing Protein-A gold) for the identification of elastin and associated microfibrillar components in connective tissues.
- distribution of 12-nm microfibrils in different tissues of the body. The following tissues were examined ultrastructrally and immunoelectron microscopically using antibodies against elastin and microfibril associated glycoprotein (MAGP). The tissues were aorta, vena cavae, muscular arteries, veins, nuchal ligament, Achilles tendon, thigh muscle, liver, lung, skin, ear cartilage, cornea, ocular zonule, periodontal ligament, spleen, kidney, placenta, umbilical cord, uterus, axillary lymph node, dura a.nd pia mater, peripheral nerve, peritoneum, small intestine, heart valve and chordae tendineae.
- histochemical, ultrastructural, immunoelectron microscopic and in situ hybridizajion study of elastofibroma in humans.antibodies a.gainst elastin (polyclonal and monoclonal), MAGP and an elastin c-DNA probe were used. The aim was to understand the pathogenesis of elastofibromas.
- study of the distribution of elastic tissues in ductal carcinoma of the human breast in order to understand the role of desmoplastic reaction in ductal carcinomas of the breasts. (using histochemical, ultrastuctural and immunoelectron microscopic)
- establishment of a human skin grafted nude mouse model to study the long-term effects of ultraviolet light irradiation on the human skin.
- developmental and age associated changes in the elastic tissues of the bovine periosteum. A histochemical, ultrastructural, immunoelectron microscopic and in situ hybridization study using elastin and MAGP c-DNA probes, and antibodies against elastin and MAGP.
- development of a long-term human skin grafted SCID mouse model.
- age related changes in the distribution of elastic tissues in normal human breast. A histochemical, ultrastructural, immunoelectron microscopic and in situ hybridization study using elastin and MAGP c-DNA probes and antibodies against elastin, MAGP and Type IV collagen.
- histochemical ultrastructural and immunoelectron microscopic investigation of the elastic spinal ligaments of humans affected with Marfan Syndrome using antibodies against elastin and MAGP.
- developmental and age associated changes in the matrix protein biosynthesis in bovine coronary arteries. A histochemical, ultrastructural, immunoelectron microscopic and in situ hybridization study using antibodies against elastin and MAGP, and elastin. microfibrillar and collagen c-DNA probes. Aim -to establish baseline data, in view of investigating atherosclerosis in human coronary arteries.
Other Publications of Interest:- Hope PJ, Pyle D, Daniels CB, Chapman I, Hororwitz M, Morely JE, Trayhurn P, Kumaratilake JS and Wittert F (1997) Identification of brown fat and mechanism for energy balance in marsupial, Sminthopsis crassicaudata. American Journal of Physiology 273, R161-R167.
- Gibson MA, Kumaratilake JS, and Cleary EG (1997) Immunohistochemical and ultrastructrual localisation of MP78/70 (Big -h3) in extracellular matrix of developing and mature bovine tissues. Journal of Histochemistry and Cytochemistry 45, 1683-1696
- Gibson MA, Finnis ML,Kumaratilake JS, and Cleary EG (1998) Microfibril- associated protein-2 (MAGP-2) is specifically associated with fibrillin-containg microfibrils, but exhibits more restricted patterns of tissue localization and developmental expression than it's structrual relative MAGP-1. Journal of Histochemistry and Cytochemistry 46, 871-885
email: Jaliya.Kumaratilake@adelaide.edu.au
Contact Jaliya: jaliya.kumaratilake@adelaide.edu.au
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